Belen Rombola-Caldentey
Stuttgart,Germany
Summary
Talented Researcher with industry-focused biotechnology experience in molecular and cell biology research, as well as a strong history of teaching, peer-reviewed publication and presentations.
Overview
10
10
years of professional experience
Work History
RESEARCH SCIENTIST
Genus PLC
10.2022 - 09.2023
- Designed and implemented platforms to investigate the interplay between sperm cells and their environment. Formulated and executed experimental plans to enhance cell health and improve sperm quality at the culmination of the sexing process.
- Maintained precise records of experiments and data analyses.
- Developed methods to assess spermatozoa capacitation using multicolor flow cytometry, fluorometric, and colorimetric methods based on plate-reader and confocal microscopy methods.
- Enhanced outcomes of the Stem Cell group across various projects by optimizing iPSC cells' growth conditions under different conditions.
POSTDOCTORAL RESEARCH FELLOW, Universität Hohenheim
11.2018 - 10.2021
- Designed and conducted research experiments, coordinated lab meetings, ensured adherence to safe laboratory protocols, and liaised with external scientists
- Co-supervised and mentored BSc and MSc students in their research and thesis activities
- Delivered practical and oral lecture sessions and displayed exceptional proficiency in sample testing, lab assays & techniques, data analysis, lab equipment, accurate reporting and documentation
- Key Achievements and Contributions:
- Instrumental role in characterizing the function and localization of Barley’s vacuolar transporters by conservation analysis, tridimensional-structure prediction, and heterologous expression in yeasts
- Utilized western-blotting and colorimetric methods for isolation and detection of proteins
- Successfully adapted and optimized protocols to isolate vacuole-enriched vesicles from yeasts
- Established protocol to measure enzymatic activity with plate reader and ACMA in the membrane of isolated vesicles of yeasts
- Designed and developed constructs – CRISPR/Cas; Agrobacterium and Yeast Expression; and E. Coli expression and cloning constructs.
PRE-DOCTORATE FELLOW, Cartuja IBVF
02.2014 - 02.2018
- Leveraged bioinformatics tools and databases for enhancing protein characterization assays, involving protein- protein interaction with Y2H and protein modeling
- Applied cloning techniques such as GreenGate (Golden Gate), Classical Cloning, and Gateway
- Showcased research results at national/international congresses through oral presentations and posters
- Awarded two mobility pre-doctoral research grants
- Key Achievements and Contributions:
- Cloned, established and characterized 12 alleles of Arabidopsis thaliana NHX1 protein by single- nucleotide point mutations of genes to detect essential and regulatory residues/domains by conservation approach and tridimensional modeling using PyMol
- Cloned and expressed the mutant alleles in different plasmid and expression systems to evaluate them in Yeast, E.coli, and Nicotiana benthamiana as heterologous expression systems
- Successfully implemented techniques for measuring intracellular pH in different organelles and plant tissues to yeasts, using ratiometric fluorophores (pH-GFP, BCECF) and confocal microscopy
- Led co-immunoprecipitation of proteins expressed in Nicotiana benthamiana and E. Coli, and detected with western-blot technique.
RESEARCH ASSISTANT
Plant Biotechnology and Genomics, UPM-INIA
08.2013 - 02.2014
- Refined theoretical and practical knowledge of bioinformatics tools and programs for generating synthetic DNA constructs, online DNA, and protein databases
- Developed and conducted plant and molecular biology-based assays, executed experiments, analyzed data, interpreted and reported results, and optimized new lab protocols
- Key Achievements and Contributions:
- Successfully authored and published one journal article in ‘The Plant Cell’ as well as published several presentations delivered at national and international congresses
- Investigated hormone signaling and germination in A. Thaliana and measured luminescence activity in-vivo in plant seeds by leveraging special hardware and software
- Mastered the use of Gateway technology and classical cloning, yeast two- and one-hybrid assays, library screening, and BiFC in N. Benthamiana
- Acquired expertise in basic plant work (in-vitro growth, propagation, transformation, transient expression and crossings) and essential molecular biology techniques such as (PCR, qPCR), RNA, and DNA isolation.
Education
Ph.D. - Molecular And Cell Biology
Universidad De Sevilla
Seville, Spain02.2019
Master of Science in Agroforestry Biotechnology -
Universidad Politécnica De Madrid, Polytechnic University
Madrid, Spain07.2013
Bachelor of Science in Biotechnology -
Universidad Pablo De Olavide
Seville, Spain09.2010
Skills
- Molecular & Cell Biology Techniques
- DNA Cloning / Genome Editing
- DNA, RNA, and Protein Preparation / Isolation
- Tridimensional-Structure Prediction
- Heterologous Expression
- Fluorescence Microscopy
- Bioinformatics Tools & Databases
- Assay Design & Development
- PCR, ddPCR, qPCR
- Good Laboratory Practice (GLP)
- Result Interpretation
- Flow Cytometry
- Protein Biochemistry
- Gene Regulation
- Data Analysis
- Molecular Biology
- Lab Equipment Operation
- Reporting Requirements
- Assay Development
- Scientific Writing
- Laboratory Safety Protocols
- DNA Cloning
- Experimental Design
- Solutions and Dilutions Preparation
- Lab Experimentation
- Internet Research
- Bioinformatics
- CRISPR
- Experiment Design
- DNA Extraction
- Laboratory Equipment Functions
- Complex Problem-Solving
- Experiment Protocol Development
- Active Listening Skills
- Protein Purification
- Reading Comprehension
- Documentation Expertise
- Self-Driven and Motivated
- Spectroscopy
Timeline
RESEARCH SCIENTIST
Genus PLC
10.2022 - 09.2023
POSTDOCTORAL RESEARCH FELLOW, Universität Hohenheim
11.2018 - 10.2021
PRE-DOCTORATE FELLOW, Cartuja IBVF
02.2014 - 02.2018
RESEARCH ASSISTANT
Plant Biotechnology and Genomics, UPM-INIA
08.2013 - 02.2014
Ph.D. - Molecular And Cell Biology
Universidad De Sevilla
Master of Science in Agroforestry Biotechnology -
Universidad Politécnica De Madrid, Polytechnic University
Bachelor of Science in Biotechnology -
Universidad Pablo De Olavide
Publications
Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signaling in the epidermis through the L1 box cis-element, Belén Rombolá-Caldentey Paloma Rueda-Romero, Raquel Iglesias-Fernández, Pilar Carbonero, Luis Oñate-Sánchez, The Plant Cell, 26, 7, 07/2014, 2905-2919, 10.1105/tpc.114.127647 Structure-Guided Identification of Critical Residues in the Vacuolar Na+,K+/H+ Antiporter NHX1 from Arabidopsis thaliana, Belén Rombolá-Caldentey, Mendoza, Imelda; Quintero, Francisco J.; Pardo, José M., bioRxiv, 05/2022, 10.1101/2022.05.18.492413 Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway, Belen RomboláCaldentey; Andrés, Z.; Waadt, R.; Quintero, F.J.; Schumacher, K.; Pardo, J.M., Int. J. Mol. Sci., 02/2023, 24, 3549, 10.3390/ijms24043549
Publications
Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signaling in the epidermis through the L1 box cis-element, Belén Rombolá-Caldentey Paloma Rueda-Romero, Raquel Iglesias-Fernández, Pilar Carbonero, Luis Oñate-Sánchez, The Plant Cell, 26, 7, 07/2014, 2905-2919, 10.1105/tpc.114.127647 Structure-Guided Identification of Critical Residues in the Vacuolar Na+,K+/H+ Antiporter NHX1 from Arabidopsis thaliana, Belén Rombolá-Caldentey, Mendoza, Imelda; Quintero, Francisco J.; Pardo, José M., bioRxiv, 05/2022, 10.1101/2022.05.18.492413 Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway, Belen RomboláCaldentey; Andrés, Z.; Waadt, R.; Quintero, F.J.; Schumacher, K.; Pardo, J.M., Int. J. Mol. Sci., 02/2023, 24, 3549, 10.3390/ijms24043549
Publications
Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signaling in the epidermis through the L1 box cis-element, Belén Rombolá-Caldentey Paloma Rueda-Romero, Raquel Iglesias-Fernández, Pilar Carbonero, Luis Oñate-Sánchez, The Plant Cell, 26, 7, 07/2014, 2905-2919, 10.1105/tpc.114.127647 Structure-Guided Identification of Critical Residues in the Vacuolar Na+,K+/H+ Antiporter NHX1 from Arabidopsis thaliana, Belén Rombolá-Caldentey, Mendoza, Imelda; Quintero, Francisco J.; Pardo, José M., bioRxiv, 05/2022, 10.1101/2022.05.18.492413 Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway, Belen RomboláCaldentey; Andrés, Z.; Waadt, R.; Quintero, F.J.; Schumacher, K.; Pardo, J.M., Int. J. Mol. Sci., 02/2023, 24, 3549, 10.3390/ijms24043549
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